BIOGENIC SYNTHESIS OF COBALT OXIDE NANOPARTICLES USING Coleus Amboinicus LEAFEXTRACT AND ITS ANTIBACTERIAL PROPERTIES
The physicochemical methods used for nanoparticle synthesis frequently involve toxic chemicals and generatehazardous by-products. Consequently, the scientific community continues to explore alternative nanoparticlesynthesis strategies that are less toxic, environmentally benign, cost-effective, and sustainable. In this context, cobalt oxide nanoparticles are of particular interest owing to their broad spectrum of applications. So, green synthesismethods of these nanoparticles are eco-friendly and could prove an alternative to physical and chemical methods. Inthis study, we utilise aqueous extract prepared from the leaves of Coleus Amboinicus for the synthesis of Co₃O₄nanoparticles. The crystallite size of synthesised nanoparticles from XRD studies is found to be around 8.378nm. The biosynthesised nanoparticles are characterised using various analytical techniques, including FTIR, XRD, SEM- EDX, and UV-Visible spectroscopy. The antibacterial activity of the synthesised Co3O4 nanoparticles (NPs) wasevaluated against a panel of Gram-positive and Gram-negative bacteria. Microbial growth is found to be adverselyinhibited by both cobalt nitrate salt solution and Co3O4 Nps, with the Co3O4 Nps exhibiting notably higher antimicrobial activity compared to the metal salt solution.
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