Author: S. Yuliasmi, Muchlisyam, J. Reveny and S. Gea

DOI: http://doi.org/10.31788/RJC.2026.1939645

A separation-free UV–Vis spectrophotometric method via a mean-centered ratio spectra methodology (MCR) approach was developed and validated for the simultaneous determination of profenofos, diazinon, and chlorpyrifosin laboratory-prepared ternary pesticide mixtures. The method showed high analytical efficiency, featuringhighlinearity (r = 0.9973–0.9999), high recovery values (103.56–107.92%), and low sensitivity limits (LOD: 0.695–19.42 µg/mL; LOQ: 2.31–64.75 µg/mL). Sequential ratio transformations followed by mean-centering effectivelyresolved the substantial spectral overlap among the three organophosphates, enabling selective quantificationwithout chromatographic separation. The validated MCR–UV–Vis procedure thus provides a rapid, solvent-efficient, and cost-effective alternative to traditional chromatographic techniques and is suitable for the routine determinationof multicomponent pesticide mixtures.

Keywords: Mean Centering of Ratio (MCR), UV–Vis Spectrophotometry, Organophosphate Pesticides, Profenofos, Diazinon, Chlorpyrifos, Multiresidue Analysis