Author: H.P. Spoorthy, N. Vijendra Kumar, D. Geetha, N.D. Rekha, G. Karthik, B.K.Kendagannaswamy and L. Mallesha

DOI: http://doi.org/10.31788/RJC.2026.1939803

The biosynthesis of AuNPs was performed efficiently using the bacteria Azospirillum brasilense, making it a greenmethod for producing gold nanomaterials. The characterisation of AuNPs was performed through UV-visiblespectroscopy, FT-IR, SEM, and XRD analysis, which showed that AuNPs are stable spherical particles withaparticle size of about 39 nm. Biological studies found that AuNPs displayed notable antibacterial, anti-inflammatory, anti-proliferation, and anti-angiogenesis properties. Remarkably, the AuNPs exhibited a moderate phospholipase A2(PLA2) inhibitory capacity, when compared to traditional drug diclofenac. In addition, the nanoparticles alsoshowed strong anti-angiogenesis ability. Overall, these results indicate the excellent bioactivity of biosynthesisedAuNPs. This study establishes a foundation for the development of eco-friendly, nanotechnology-based therapeuticswith broad-spectrum efficacy.

Keywords: A. Brasilense, Gold Nanoparticles, Antimicrobial, Anti-Inflammatory, Antiproliferative, Anti- Angiogenic.